Just gonna throw this out there, PLEASE dont run rubber the whole way! Hard line is so cheap and easy to use its insane not to use it, you can get 25' and 50' rolls from summit for so cheap its crazy, just get the aluminum setup and be done with it. If you get the aluminum you can run tube and nuts on each end after flaring them to attach to the gas tank and get some of those rubber insulated clamps from home depot, I just drilled and tapped my frame for some really small bolts (maybe 1/4" will work also).
If your deadset on the rubber and all the possible headaches that come with it (ask me how I know and why I will NEVER run all rubber on a diesel again) then get something high quality (read expensive) and stay away from aeroquip pushlock hose it dry rots with diesel fuel. You can run push lock fittings on the ends, go with some 3/8" feed line with -6AN or to save money go to a plumbing supply and get standard steel -6 JIC fittings for the returne 5/16" line will be fine and you can run a -4 an or jic fitting, you can also run 3/8 for both sending and return if you want.
For the through the frame part, it will help you to run an or jic fittings agian. You can buy a "bulkhead fitting" from summit, jegs or any large plumbing supply that is fairly long and double male with a jam nut to hold it in place.
http://www.google.com/imgres?imgurl...bnw=124&prev=/images?q=jegs+bulkhead+fitting&um=1&hl=en&sa=G&ndsp=18&tbs=isch:1
Also please please please stay away from hose clamps as much as possible to save yourself some headache, I had a problem with my ford/ isuzu setup when I first got it running, it would start then die I chased that problem for 2 days with all good tight connections, finally after going through it all I tighted the right fitting till it was about to break the clamp and it worked for a while then once it loosened backup I had the same problem. After going to all AN or JIC fittings that I could tighten up with a pair of wrenches I never had that problem again.